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KMID : 0616120160470010007
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Korean Journal of Pharmacognosy
2016 Volume.47 No. 1 p.7 ~ p.11
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Anti-inflammatory Constituents of the Aerial Parts of Trichosanthes kirilowii in BV2 Microglial Cells
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Li Xiao Jun
Kim Kwan-Woo
Ko Won-Min
Kim Dong-Cheol
Yoon Chi-Su
Liu Xiang Qian
Kim Jong-Su
Jang Kyu-Gwan
Kang Dae-Gill
Lee Ho-Sub
Oh Hyun-Cheol
Kim Youn-Chul
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Abstract
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The aerial part of Trichosanthes kirilowii Maxim. (Cucurbitaceae), has long been used in traditional Korean and Chinese medicines for the treatment of heatstroke. We isolated and identified three flavones, luteolin-7-O-β-D-glucopyranoside(1), luteolin-4′-O-β-D-glucopyranoside(2), luteolin(3) from its methanolic extract. In the present study, we found that luteolin attenuates the lipopolysaccharide(LPS)-induced inflammation in BV2 microglial cells. Luteolin significantly inhibited LPS-induced production of pro-inflammatory mediators such as nitric oxide(NO) and prostaglandin E2(PGE2) in BV2 microglia in a concentration-dependent manner without cytotoxic effect. Luteolin dose-dependently suppressed the protein expression of inducible nitric oxide synthase(iNOS) and cyclooxygenase-2(COX-2). In addition, luteolin also showed significant induction of heme oxygenase(HO)-1. These results suggest that both the aerial part of T. kirilowii and luteolin may be good candidates to regulate LPS-induced inflammatory response.
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KEYWORD
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Trichosanthes kirilowii , Luteolin , Anti-inflammation , BV2 cells , Heme oxygenase-1
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